Pdf antidiabetic potential of caralluma europaea against. Alloxan induced diabetes in mice protocol diabetestalk. On the 4th day of alloxan induced diabetes, some of the mice were given insulin and glucose to prevent hypoglycemia. Effects of alloxan diabetes, antiinsulin serum diabetes. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Antidiabetic evaluations of some traditional plants in. Sensitization to alloxan induced diabetes and pancreatic cell apopto. Alloxaninduced diabetes is a model of type 1 dm, but type 2 dm is a more common risk factor for cvd. Antioxidant and hypoglycemic effects of tithonia diversifolia aqueous leaves extract in alloxaninduced diabetic mice abstract diabetes is a chronic disease cause the metabolic disorder and associated with serious complications. Diabetes follows the alloxaninduced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet.
Antidiabetic effects of rhse were evaluated in both the rat insulinoma1 cell line ins1 and diabetic icr mice induced by intraperitoneal ip injection of alloxan. Antioxidant and hypoglycemic effects of tithonia diversifolia. Protective role of three vegetable peels in alloxan induced diabetes mellitus in male mice. Pdf efficacy of chitooligosaccharides for the management of. Volume 1802, issue 2, february 2010, pages 240246 sensitization to alloxaninduced diabetes and pancreatic cell apoptosis in acatalasemic mice author links open overlay panel yokokikumotoa hitoshisugiyamaab human acatalasemia may be a risk factor for the development of diabetes mellitus. Journal of medicinal plants research hypoglycemic effect. Efficacy of chitooligosaccharides for the management of diabetes in alloxan induced mice. Alloxaninduced diabetes, a common model for evaluating the. Alloxan treatment 10 mm increased cellular reactive oxygen species ros levels in the. Alloxan elicited pancreatic lipid peroxidation which precedes the appearance of hyperglycemia in balbc mice. Blank group and model group are given the same volume of saline.
Alloxan induced diabetes model diabetes was induced by a single intraperitoneal injection of 150 mgkg monohydrated alloxan sigma, st. The role of silymarin in prevention of alloxaninduced. Diabetes follows the alloxan induced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet beta cell, producing minimal effects on other structures. Nox2 deficiency protects against streptozotocininduced. Hyperglycaemia was induced in fasted wistar rats with alloxan 150 mgkg body weight. Pdf the mechanism of alloxan and streptozotocin action. Molar glucose, 30 mlkg, was given subcutaneously at 34, 4, and. Aloesin up394 was formulated with an aloe vera inner leaf gel powder polysaccharide qmatrix to yield a composition designated up780. Alloxan elicites pancreatic lipid peroxidation which precedes the appearance of hyperglycemia in balbc mice. Diabetes was induced by the intraperitoneal injection of alloxan.
Frontiers antihyperglycemic and antihyperlipidemic. It was observed that the mice given a diet containing 0. Altered glucose metabolism and hypoxic response in alloxan. The mechanism of alloxan and streptozotocin action in b cells. Cinnamon extract on alloxan induced diabetes in experimental mice. We studied the effects of the free radical scavenger, silymarin on balbc mice pancreas, the effect of this flavonoid on pancreatic, hepatic and blood glutathione together with. Effects of ece on body weights a and fasting blood glucose levels b in normal and alloxaninduced diabetic rats. Diabetes was induced using intraperitoneal injection of alloxan 100 mgkg, 0. Hypoglycemic effect of lagerstroemia speciosa pers. Mice with two to threefold increase in blood sugar levels were considered diabetic and used for further tests. In this study the effects of petroleum ether and ethanol extracts of the seeds of citrullus lanatus on blood glucose levels in alloxan induced in mice have been investigated. In a preliminary study, effects of ethanolic extracts of cucurbita pepo, cucumis sativus and praecitrullus fistulosus peels were studied at 250 and 500 mg kg.
Such effect was concomitant with an enhanced histological features of pancreatic lesions, leading to a possible pancreatoprotective potential of the plant. Nicotinamide protected nmri mice against alloxan induced hyperglycemia when given 10 min before, but not 10 min after, the injection of the drug. Research design and methods five groups of micewildtype wt, nox2. Hypoglycemic effects in alloxaninduced diabetic rats of the. Effect of oral administration of lactobacillus casei on.
This study investigated the protective effect of a liquid rice hull smoke extract rhse against diabetes in alloxaninduced diabetic mice. Amaranthus dubious on alloxan induced diabetic mice. Alloxan is a cyclicurea derivative, which is a highly effective, diabetogenic agent used widely as a tool to induce experimental diabetes. Since this claim has not been investigated scientifically, the aim of this study was to evaluate the antidiabetic effect and phytochemical screening of the aqueous and 70% ethanol extracts on alloxaninduced diabetic mice. The effects of lactobacillus casei lc on the onset of alloxan axn. Lakshmi bhavani department of botany, university college of science saifabad, osmania university, hyderabad 500004, telangana, india. Metabolomics analysis of alloxaninduced diabetes in mice. The present study aimed to investigate the potential antidiabetic and hypolipidemic activity of tea polysaccharides in mice with alloxaninduced diabetes. Alloxaninduced diabetes causes morphological and ultrastructural. The most plausible explanation for induction of diabetes due to alloxan is that following its. Hyperglycemia was induced by the injection of 150 mgkg i. Periodontal disease in diabetic patients presents higher severity and prevalence.
Data provided through the present investigation sustained an obvious and potent antihyperglycemic activity of c. Antihyperglycemic potential of teak seeds in alloxan induced diabetic rats html full text. Alloxaninduced diabetes model diabetes was induced by a single intraperitoneal injection of 150 mgkg monohydrated alloxan sigma, st. This study evaluated the longterm effects of alloxan induced diabetes in rat liver. Nicotinamide protected nmri mice against alloxaninduced hyperglycemia when given 10 min before, but not 10 min after, the injection of the drug. Apr 06, 2018 volume 1802, issue 2, february 2010, pages 240246 sensitization to alloxan induced diabetes and pancreatic cell apoptosis in acatalasemic mice author links open overlay panel yokokikumotoa hitoshisugiyamaab human acatalasemia may be a risk factor for the development of diabetes mellitus. Protective role of three vegetable peels in alloxan. We have studied the effects of the free radical scavenger, silymarin on balbc mice pancreas, the effect of this flavonoid on pancreatic, hepatic and blood glutathione together with.
Research article antidiabetic activity of polyphyto. Antidiabetic activity and phytochemical screening of. The mechanisms of alloxan and streptozotocininduced. Effect of aspergillus awamorifermented burdock root on. In research it is used for induction of type 1 diabetes.
Antihyperglycemic effects of fermented and nonfermented mung. Although their cytotoxicity is achieved via different pathways, their mechanisms of beta cell selective action are identical. Alloxan is a urea derivative which causes selective necrosis of the. However, in absence of aggressive stimuli such as ligatures, the influence of diabetes on. Diabetes was induced with single intraperitonial injection of alloxan monohydrate 200 mgkg body weight and animals were treated with. On the 4th day of alloxaninduced diabetes, some of the mice were given insulin and glucose to prevent hypoglycemia. Such effect was concomitant with an enhanced histological fea tures of pancreatic lesions, leading to a possible pancreato.
Alloxan is selectively toxic to insulinproducing pancreatic beta cells because it preferentially accumulates in beta cells through uptake via the glut2 glucose transporter. In the present study activities of liver enzymes such as glucokinase, glucose 6 phosphatase and fructose 1 6diphosphatase were also determined. Administration of extract to alloxan induced diabetic mice alloxan induced diabetic mice were. The mechanism of alloxan and streptozotocin action in b. Hyperglycaemic rats were treated with or without oral. Attenuation of diabetic hepatopathy in alloxaninduced diabetic mice. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. The positive control group was given diamicron suspension. Animals models for studying diabetes mellitus etuk, e. After 12 h, a 10% glucose solution was offered to the animals to prevent hypoglycemia 27,28. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t.
Alloxan monohydrate 150 mgkg bw prepared in citrate buffer. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation. Alloxan monohydrate induces diabetes in animals, although streptozotocin. When the tenth, twentieth, thirtieth day of the administration, measured the blood. Effect of curcumin on diabetes in an alloxan mice model. Reduced nociceptive responses in mice with alloxan induced. Effect of aloe vera leaf extract on alloxan induced. As existing treatment is inadequate, research for better drugs should be carried out. Effect of aloe vera leaf extract on alloxan induced diabetes. The mechanisms of alloxan and streptozotocin induced diabetes.
Protective role of three vegetable peels in alloxan induced. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. The mice were randomly assigned into six different groups 8. Although lagerstroemia speciosa has been shown to produce hypoglycemic effects in some mice models of diabetes, there are no reports of the effects of this substance in. Then, alloxan 200 mgkg of body weight was administrated to each mouse.
May 24, 2014 insulin dependent diabetes was induced by administering a single intraperitoneal injection of alloxan monohydrate at a dose of 150 mgkg to cd1 mice. Department of zoology, sadguru gadage maharaj college, karad 415110, india available online. International journal of biological macromolecules 2018 v. The antidiabetic activity of the total lignan from the plant fructus arctii, used in china for the control of diabetes, was investigated in models of alloxan. Diabetes was induced in rats by giving intraperitonial injection of single dose 85mgkg of freshly prepared alloxan monohydrate dissolved in saline solution. Mice administered a single intraperitoneal injection of alloxan monohydrate at a dose of 150 mgkg showed progression of typei diabetes symptoms figure 2. Alloxan has been administered in single or multiple doses, through different routes intraperitoneal. The hitherto unknown glucose regulating role of three vegetable peels from cucurbitaceae family was evaluated.
It has been widely used to induce experimental diabetes in animals such as rabbits, rats, mice and dogs with different. Blood glucose levels and body weights of control and diabetic mice were monitored. Research article alloxan induced diabetes mellitus in wistar. In animals, it can be induced by partial pancreatectomy or by the administration of diabetogenic drugs such as alloxan, streptozotocin, ditizonx and antiinsulin serum 10. Antihyperglycemic effects of fermented and nonfermented. Various studies have shown that diabetes mellitus is associated with increased the formation of free radicals and. Objective the role of nox2containing nadph oxidase in the development of diabetes is not fully understood. For the management of the carbohydrate metabolism in diabetes by the cos, the amount of glucose in blood along with. It has been proposed that lipid peroxidation caused by free radicals may be involved in alloxan induced diabetes mellitus. Blood glucose lowering activity of aloe based composition. Frontiers antihyperglycemic and antihyperlipidemic effects.
Phytochemical screening, antihyperglycemic and antihyperlipidemic effects of aqueous extracts of l. A diet containing the 5% fermented burdock powers was prepared to examine effect of the burdock diet on alloxaninduced mouse diabetes. Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals. Alloxaninduced diabetes and bone repair 373 issn 0100. This study was conducted to compare the antidiabetic effect of. The diabetic mice treated were with ppaqu 500mgkg, p. Sensitization to alloxaninduced diabetes and pancreatic cell apopto. Diabetes mellitus is a collection of disorder which results from either lack of insulin or factors, which interfere with the action of this hormone. They were given 5% of glucose in drinking water after 1 hr to encounter any initial hypoglycemia. Hypoglycemic activity of ethanolic leaf extract of. Preparation of diabetic mice diabetes was induced by the administration of alloxan monohydrate to mice according to syiem et al. Diabetic mice attained the blood sugar levels ranging from 220mgdl to. Pdf alloxaninduced diabetes, a common model for evaluating the.
In 1838, wohler and liebig 1 synthesised a pyrimidine derivative, which they later called alloxan 2, 3. Phytochemical screening and antidiabetic effect of. Alloxaninduced diabetes, a common model for evaluating. Ajuga remota benth is traditionally used in ethiopia for the management of diabetes mellitus. In 1943, alloxan became of interest in diabetes research when dunn and mcletchie reported that it could induce diabetes in animals 4 as a result of the specific necrosis of the pancreatic beta cells 57. Antidiabetic potential of caralluma europaea against. The resulting insulinopenia causes a state of experimental diabetes mellitus called alloxan diabetes 4, 8, 9. Pdf the mechanism of alloxan and streptozotocin action in b. Interaction of aqueous extract of pleurotus pulmonarius.
The mechanisms of alloxan and streptozotocininduced diabetes. Alloxaninduced diabetes triggers the development of. Dose optimization of alloxan for diabetes in albino mice. Genetic and surgical models were rarely used because they required highly technical skills and the percentage of animals lost during the procedures were higher. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that. Pretreatment in vivo with nicotinamide induced hyperglycemia at the time of alloxan injection, and this could account for the protective action of nicotinamide against alloxan diabetes. Antidiabetic effects of rice hull smoke extract in alloxan. Molar glucose, 30 mlkg, was given subcutaneously at 34, 4, and 3. Mice became diabetic after 24 hr and were maintained by supplementation of alloxan on i st, 7th, 14th, 21 st and 28th days. Effect of aloe vera leaf extract on alloxan induced diabetes in young mice j. Group 1 was the normal control mice that received distilled water as placebo while groups 26 were diabetic mice. Antidiabetic effect of ethanolic leaf extract of phyllanthus.
This causes an insulindependent diabetes mellitus called alloxan diabetes in these animals, with characteristics similar to type 1 diabetes in humans. We hypothesized that nox2 deficiency decreases reactive oxygen species ros production and immune response and protects against streptozotocin stzinduced. Thus, the present study aimed to elucidate the hypoglycemic effects of l. Fresh liver weight and its relationship with body weight were obtained, and liver tissue was. Metabolomics analysis of alloxan induced diabetes in mice using uplcqtofms after crassostrea gigas polysaccharide treatment author. We analyzed specimens at only five weeks after inducing diabetes and thus timecourse changes in vascular metabolism during atherogenesis were not investigated. U department of pharmacology, college of health sciences, usmanu danfodiyo university. We report herein the findings of this research work. Growth and treatment of ehrlich tumor in mice with alloxan. Research on the effect of curcumin on diabetes in an alloxan mice model. The extract of bitter melon, chirata and cinnamon were tested for its efficacy in alloxan at a dose 120mgkg of body weight induced diabetic mice. The effect of aepe leaf extract on blood glucose, insulin, hemoglobin and.
Zhao, guanhua, hou, xilong, li, xingyao, qu, min, tong, changqing, li, wei source. Mice fed the burdock diet and the control diet for 14 weeks. The antidiabetic activity of total lignan from fructus. Rats were made to fast prior to alloxan administration. The present study evaluates the effects of chitooligosaccharides cos for the management of alloxan induced diabetes in mice. Pdf efficacy of chitooligosaccharides for the management. Antioxidant and hypoglycemic effects of tithonia diversifolia aqueous leaves extract in alloxan induced diabetic mice abstract diabetes is a chronic disease cause the metabolic disorder and associated with serious complications. Diabetes was induced by sc injection of alloxan monohydrate tloba chern ie, indo austranal co.
Thirty nondiabetic control rats nc and 30 untreated. Cinnamon administered group show restoration in both biochemical and histol ogical parameters of kidney of mice. Alloxan and streptozotocin are widely used to induce experimental diabetes in animals. Antidiabetic activity and phytochemical screening of extracts. Alloxan and streptozotocin are the most prominent diabetogenic chemicals in diabetes research.
The greater the dose, the less mice accepted the tumor. It has been successfully induced in a variety of animal species. This study evaluated the longterm effects of alloxaninduced diabetes in rat liver. Alloxaninduced diabetes delays repair in a rat model of. Insulin injection into diabetic tumorbearing mice promoted the tumor growth. It has been widely used to induce experimental diabetes in animals such as rabbits, rats, mice and dogs with different grades of disease severity by varying the dose of alloxan used 8. Alloxan has been administered in single or multiple. The cytotoxic action of both these diabetogenic agents is mediated by reactive oxygen species, however, the source of their generation is different in the case of alloxan and. Phytochemical screening and antidiabetic effect of extracts. High, medium and low treatment groups were given different doses of curcumin solution. Thirty nondiabetic control rats nc and 30 untreated diabetic ud rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks.
Dec 18, 2007 alloxan has two distinct pathological effects. A correlative study with antihyperlipidemic and antioxidative activity. As seen from the data in figure 2, fasting blood glucose levels were reduced versus vehicle treatment following two weeks of oral treatment with glyburide 39. In this study the effects of petroleum ether and ethanol extracts of the seeds of citrullus lanatus on blood glucose levels in alloxaninduced in mice have been investigated. After cancer and cardiovascular diseases, diabetes is the third most common cause of death. Alloxaninduced diabetes and bone repair 373 issn 0100879x. Since this claim has not been investigated scientifically, the aim of this study was to evaluate the antidiabetic effect and phytochemical screening of the aqueous and 70% ethanol extracts on alloxan induced diabetic mice. The mechanism of their action in b cells of the pancreas has been intensively investigated and now is quite well understood. Effects of alloxan diabetes, antiinsulin serum diabetes, and. The antidiabetic effect was conducted on swiss albino mice. We studied the effects of the free radical scavenger, silymarin on.
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